Table 2.

Data for refolding of S6 mutants in M Na₂SO₄

Mutant	log KCwater*	mCGdmCl, M−1	log kf3M GdmCl, s−1*	mfGdmCl, M−1	ΔΔGD−N, kcal⋅mol−1†	ΦC‡
Wild-type	4.37  ± 0.06	−1.47  ± 0.01	1.82  ± 0.02	−1.29  ± 0.02	≡ 0
VA6	4.01  ± 0.08	−1.54  ± 0.02	0.80  ± 0.02	−1.44  ± 0.02	3.56  ± 0.16	0.14  ± 0.1
VA8	2.78  ± 0.07	−1.17  ± 0.02	0.69  ± 0.02	−1.18  ± 0.02	3.74  ± 0.17	0.59  ± 0.02
VA9	3.64  ± 0.10	−1.45  ± 0.02	1.64  ± 0.02	−1.27  ± 0.03	1.65  ± 0.08	0.61  ± 0.02
LA21	4.30  ± 0.13	−1.50  ± 0.03	1.76  ± 0.03	−1.29  ± 0.03	0.57  ± 0.03	0.19  ± 0.05
EQ22	5.11  ± 0.18§	−1.67  ± 0.07	1.82  ± 0.07‡	−1.34  ± 0.05	1.60  ± 0.08	−0.64  ± 0.03
IA26	3.33  ± 0.06	−1.40  ± 0.02	1.05  ± 0.02	−1.22  ± 0.02	2.76  ± 0.13	0.53  ± 0.01
LA30	4.38  ± 0.15	−1.58  ± 0.04	1.18  ± 0.04	−1.42  ± 0.04	3.26  ± 0.15	−0.00  ± 0.01
YA33	4.19  ± 0.06	−1.45  ± 0.01	1.77  ± 0.02	−1.26  ± 0.02	0.38  ± 0.02	0.67  ± 0.03
AG35	4.78  ± 0.07	−1.55  ± 0.02	1.83  ± 0.02	−1.39  ± 0.02	0.84  ± 0.04	−0.67  ± 0.02
VA37	4.14  ± 0.09	−1.52  ± 0.02	1.53  ± 0.02	−1.35  ± 0.02	2.50  ± 0.19	0.13  ± 0.01
VA40	3.97  ± 0.10	−1.45  ± 0.02	1.64  ± 0.03	−1.29  ± 0.03	0.59  ± 0.04	0.96  ± 0.04
IA48	4.04  ± 0.06	−1.43  ± 0.01	1.79  ± 0.02	−1.27  ± 0.02	0.77  ± 0.04	0.61  ± 0.02
FA60	4.19  ± 0.07	−1.51  ± 0.02	1.71  ± 0.02	−1.29  ± 0.02	1.21  ± 0.06	0.21  ± 0.01
VA65	3.89  ± 0.10	−1.52  ± 0.03	1.17  ± 0.02	−1.37  ± 0.03	2.95  ± 0.13	0.23  ± 0.01
MA67	4.11  ± 0.09	−1.49  ± 0.02	1.40  ± 0.02	−1.33  ± 0.02	2.30  ± 0.11	0.16  ± 0.01
LA75	4.11  ± 0.11	−1.55  ± 0.03	1.31  ± 0.03	−1.27  ± 0.03	1.92  ± 0.10	0.19  ± 0.01
LA79	3.41  ± 0.09	−1.32  ± 0.02	1.23  ± 0.02	−1.15  ± 0.03	4.39  ± 0.20	0.30  ± 0.01
VA85	5.24  ± 0.23§	−1.84  ± 0.09	1.49  ± 0.01‡	−1.13  ± 0.04	2.97  ± 0.15	−0.40  ± 0.01
VA88	3.57  ± 0.04	−1.33  ± 0.01	1.53  ± 0.10	−1.18  ± 0.01	1.93  ± 0.09	0.58  ± 0.02
VA90	3.57  ± 0.06	−1.37  ± 0.01	1.55  ± 0.02	−1.23  ± 0.02	2.44  ± 0.12	0.46  ± 0.01

*Data fitted to Eq. 2 except where indicated. 

^†Calculated from equilibrium denaturation data (16). 

^‡Calculated according to Eq. 5. 

^§Data fitted to Eq. 4 (triangular model).