Figure 1.

Dex inhibition of the TGF-β transcriptional activation of human PAI-1. Hep3B cells cultured in 6-well plates were transiently transfected with 0.5 μg of the reporter plasmid pPAI-1(−800/+22)-luc (A) or pTRS₆E1b-luc (B) and 0.2 μg of human GR expression plasmid pRShGRα. Cells were cultured for 24 h in the presence or absence of 50 pM TGF-β and/or 100 nM Dex. Results are presented as the mean ± SD (n = 3) of relative luciferase activity.