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. 1999 Oct 12;96(21):11776–11781. doi: 10.1073/pnas.96.21.11776

Figure 3.

Figure 3

Smad3/4 activation domain-activated transcription is specifically inhibited by Dex. Hep3B cells were transiently transfected with 0.5 μg of the reporter plasmid pG5B-luc, 0.5 μg of GAL4Smad4C (A), 10 ng of GAL4Smad3C (B), or 10 ng of GAL4VP16 (C) expression plasmids and pRShGRα. As a control, 0.5 μg of the plasmid, pSG424, which expresses the GAL4 DNA-binding domain (GAL4 (1–147) only, was transfected. Cells were cultured for 24 h in the presence or absence of 50 pM TGF-β and/or 100 nM Dex. Results are presented as the mean ± SD (n = 3) of relative luciferase activity.