Figure 5.

The C-terminal domain of GR is required for repression of transcriptional activation by TGF-β. Hep3B cells cultured in 6-well plates were transiently transfected with 0.5 μg of the reporter plasmid pTRS6E1b-luc and 0.2 μg of expression plasmids for wild-type or mutant human GR, as indicated. Cells were cultured for 24 h in the presence or absence of 50 pM TGF-β and/or 100 nM Dex. Results are presented as the mean ± SD (n = 3) of relative luciferase activity.