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Electrophoretic mobility-shift assay. The free radiolabeled C1 probe in the absence of OmpR protein is shown in lane 1 (indicated by Free C1 to the left of the lane). OmpR (8 μM) was incubated with 4 μM C1 for 3 h at RT in the absence (lane 2) or presence (lane 3) of 25 mM acetyl phosphate, and the shifted complex is indicated by OmpR/C1 Complex to the left of the gel.
