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. 1999 Oct 12;96(21):11792–11797. doi: 10.1073/pnas.96.21.11792

Figure 4.

Figure 4

Fluorescent labeling of OmpR with PyMPO. OmpR (9.5 μM) is reacted with a 50-fold excess of PyMPO for 30 min at RT in 40 mM Imidazole/1 mM EDTA, pH 7.6 (lane 1). Lane 2 shows a stimulation of PyMPO labeling in the presence of C1. OmpR (9.5 μM) is incubated with acetyl phosphate for 3 h at RT in the absence (lane 3) or presence (lane 4) of C1 DNA and then labeled with PyMPO as before. The products of the labeling reaction are separated on SDS/12% PAGE. The visible fluorescent band is the PyMPO-labeled OmpR protein.