Table 1.
The effect of DNA on OmpR phosphorylation by acetyl phosphate
| OmpR-P, percentage of total | Fold stimulation | |
|---|---|---|
| No DNA added | 45 | |
| F1 | 84 | 1.9 |
| F1 half site | 42 | 0 |
| F2 | 59 | 1.3 |
| F3 | 45 | 0 |
| F4 | 85 | 1.9 |
| F1–F2–F3 | 83 | 1.8 |
| C1 | 86 | 1.9 |
| C1 half site | 41 | 0 |
| C2 | 36 | 0 |
| C3 | 33 | 0 |
| C1–C2–C3 | 84 | 1.9 |
| pstS | 42 | 0 |
The role of individual binding sites in stimulating OmpR phosphorylation by acetyl phosphate. In the first column, the oligonucleotides present during the phosphorylation reaction are listed. In the second column, OmpR-P is expressed as a percentage of the total OmpR protein present in the reaction, as determined by C4 reverse-phase HPLC. The third column shows the stimulation caused by the presence of DNA. Phosphorylation was for 3 h as described in Materials and Methods, and the oligonucleotides were present at a 2-fold molar excess in the presence of 10 μM OmpR.