Table 1.

Thermal stabilities (T_m) of PNA·DNA and PNA·PNA complexes

PNA sequence (PNA number)	Tm, °C
	Vs. DNA*	Vs. PNA†
H-GTAGATCACT-Lys-NH2	51	68
H-GTAGDTCACT-Lys-NH2	57	71 (64)‡
H-GTDGDTCDCT-Lys-NH2	67	81 (49)§
H-Lys-GsUDGDsUCDCsU-Lys-NH2 (1495)	58	74
H-AGTGATCTAC-Lys-NH2	49	68
H-AGTGAsUCTAC-Lys-NH2	46	68
H-AGUsGAsUCsUAC-Lys-NH2	44	68
H-Lys-DGsUGDsUCsUDC-Lys-NH2 (1496)	58	74
H-Lys-GsUDGDsUCDCT-Lys-NH2 (1674)	65.5
H-Lys-AGsUGDsUCsUDC-Lys-NH2 (1672)	56
H-Lys-GsUAGDsUCDCT-Lys-NH2 (1675)	61
H-Lys-AGsUGDsUCTDC-Lys-NH2 (1673)	58
H-Lys-GsUDGGsUCDCsU-Lys-NH2 (1837)	67
H-Lys-DGUsGDCCsUDC-Lys-NH2 (1838)	66
H-Lys-DCGDCsUCDCsU-Lys-NH2 (1657)	67
H-Lys-DGsUGDGsUCGsU-Lys-NH2 (1658)	68

^*The complementary DNA oligonucleotides 5′-d(AGTGATCTAC) and 5′-d(GTAGATCACT) were used. Thermal stabilities (T_m) were measured in 10 mM sodium phosphate/100 mM NaCl/0.1 mM EDTA, pH 7.0, at a heating rate of 0.5 °C per step (0.7°C/min) (5–90°C). 

^†The complementary PNA oligomers H-AGTGATCTAC-Lys-NH₂ and H-GTAGATCACT-Lys-NH₂ were used. 

^‡Measured vs. H-AGTGAsUCTAC-Lys-NH₂. 

^§Measured vs. H-AGsUGAsUCsUAC-Lys-NH₂.