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. 1999 Oct 12;96(21):11854–11859. doi: 10.1073/pnas.96.21.11854

Figure 3.

Figure 3

Effect of cyclic DAG on the subcellular redistribution of GFP-β2-chimaerin and GFP-C246A-β2-chimaerin. (A) COS-1 cells were transfected with pEGFP-β2-chimaerin or pEGFP-C246A-β2-chimaerin. Forty-eight hours later cells were treated with B8-DL-B8 (100 μM) for different times and subjected to subcellular fractionation. Western blot analysis using an anti-β2-chimaerin antibody was performed on soluble and particulate fractions. Two additional experiments gave similar results. (B) COS-1 cells were transfected with pEGFP-β2-chimaerin (a–c), pEGFP-C246A-β2-chimaerin (d and e), or pEGFP (f and g) and treated with the DAG lactones (100 μM, 30 min) or vehicle (DMSO), as indicated in each case. Cells were fixed with 3.7% formaldehyde and visualized by fluorescent microscopy. Similar results were observed in two additional independent experiments.