Figure 6.

Growth characteristics and attenuated EMCV infectivity in U9K-AV2 cells. (A) U9K-A and U9K-AV2 cultures (each with a starting cell density of 10⁶ cells in 10 ml of medium) were followed for up to 96 hr for growth curve determination (trypan blue viable cell counting). (B) Cells (5 × 10⁶) of EMCV-infected U9K-A (48 hr p.i.) and U9K-AV2 cells were harvested for viral titration and total RNA extraction. Ten micrograms of total RNA was dot-blotted onto nitrocellulose membrane and hybridized with ³³P-random-primed EMCV-specific probes. Both viral titers and EMCV RNA axes are plotted on log₁₀ scales.