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Colony formation assay was performed with the pcDNA3-BRCA1 plasmid linearized at different sites for positive and negative control. (A) Restriction map of the plasmid used in this experiment. (B) Western blot analysis of BRCA1 expression from differentially cleaved pcDNA3-BRCA1 in Cos-7 cells. (C) Colony formation assay with linearized plasmids. ■, no BRCA1 control (HindIII–AflII digest). ▧, overexpression of BRCA1 (PvuI digest).
