Figure 6.

Identification of BRCA1-Rb interactions. (A) Schematic representation of BRCA1 fragments used for in vitro translation or GST-fusion proteins. (B) In vitro-translated [³⁵S]methionine-labeled BRCA1 fragments were incubated with GST–Rb recombinant proteins or GST alone. The complexes were collected with glutathione–Sepharose and resolved on SDS/PAGE followed by autoradiography. (C) In vitro-translated Rb or mutated Rb (C706F) proteins were used in binding to GST–BRCA1 fragments as described in B. One-fifth of the input was loaded in the IVT lane.