Figure 3.
Expression of Alg-3 in adult liver. (A) Western blot analysis of material of 3-month-old animals. The blot was stained first with PS2CTF antiserum and afterward with anti-PS1NTF. Notice that PS2CTF are expressed only in liver in the PS2−/− mice. PS1NTF are present at equal levels in PS2 wild-type and knock-out animals. (B) Membrane-enriched material from liver was prepared from E13.5 embryos and 1-week- and 3-month-old animals and was probed with antibodies specific for the carboxyterminus of PS1 (PS1CTF) or PS2 (PS2CTF). Notice that only in 3-month-old PS2−/− animals are PS2CTF fragments expressed.