Table 1.
Alignment of MSP-1 activation sequence with the predicted MT-SP1 cleavage sequence consensus
| P4 | P3 | P2 | P1 | |
|---|---|---|---|---|
| Filaggrin | R | K | R | R |
| HGF/SF | K | Q | L | R |
| MT-SP1/matriptase | R | Q | A | R |
| PAR2 | S | K | G | R |
| Trask/CDCP1/SIMA135 | K | Q | S | R |
| uPA/urokinase | P | R | F | K |
| *P1-diverse PS-SCL | K/R | K/R | S > P > G > L | K/R |
| Phage Display | K/R vs. X | X vs. K/R | Small/hydrophobic | K/R |
| Crystal Structure | K/R vs. X | X vs. K/R | Small/hydrophobic | K/R |
| Consensus | K/R vs. S/P | Q vs. K/R | Small/hydrophobic | R > K |
| *MSP-1 | S | K | L | R |
Per standard notation, P1 is designated as the amino acid N-terminal to the scissile bond with P2 being the amino acid N-terminal to P1, etc. The P4 through P1 amino acids for known substrates of MT-SP1, P1-diverse PS-SCL data, phage display data, and crystallographic determinations are displayed. An MT-SP1 consensus cleavage sequence was derived from this information. The MSP-1 activation sequence is also presented. Asterisks indicate compiled PS-SCL data or MT-SP1 cleavage sequences determined by N-terminal sequencing in this work.