Table 1.

Electroporation results at 48 hpf

	Transfection levels*
Voltage	+++	++	+	None	Dead
30 (n = 77†)	72 (94%)	3 (4%)	-	-	2 (3%)
20 (n = 16)	2	8	5	1	-
10 (n = 16)	-	-	5	11	-

Injection of 0.7 mg/ml each pHuc:GAL4/pUAS:EGFP into the lateral forebrain and ventricle was followed by two manually applied trains of five 1 ms pulses at the indicated voltage. Transfection levels were greatly decreased with voltages lower than 30 V. *Embryos were viewed dorsally under a compound fluorescence microscope and categorized according to extent of transgene expression: +++, similar to Figure 2a or higher; ++, intermediate levels; +, few enough cells to make out and count individually, fewer than 20. ^†Of 80 embryos electroporated in 2 experiments, 3 were killed by mechanical damage during removal from agarose and are not included.