Table 2.
Dilution | Analysis method | ||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | |
0.1 | 0.084 ± 0.003 | 0.11 ± 0.0033 | 0.57 ± 0.30 | 0.37 ± 0.19 | 0.75 ± 0.45 | 0.71 ± 0.63 | 0.12 ± 0.022 |
1 | 1 ± 0.019 | 1 ± 0.017 | 1 ± 0.11 | 1 ± 0.20 | 1 ± 0.12 | 1 ± 0.10 | 1 ± 0.018 |
10 | 14 ± 0.680 | 10 ± 0.35 | 242 ± 206 | 83 ± 52 | 15 ± 5.5 | 48 ± 1.0 | 10 ± 1.1 |
(1)CT method assuming constant amplification efficiency equal to 1 [12].
(2) CT method with amplification efficiency estimated from a dilution series [14].
(3) Amplification efficiency estimated at two product yield thresholds [15].
(4) Amplification efficiency estimated with LinRegPCR software [13].
(5) Amplification efficiency estimated with the Tichopad et.al. approach [16].
(6) Amplification efficiency estimated from the model proposed by Liu et.al. [17].
(7) Our model based real-time PCR analysis method (MoBPA).