Table 2.

Analysis of real-time PCR results with similar amplification efficiency among samples. Data represent the quantification of dilutions 0.1 and 10 as the mean ± SEM for 12 experiments performed in triplicate.

Dilution	Analysis method
	1	2	3	4	5	6	7
0.1	0.084 ± 0.003	0.11 ± 0.0033	0.57 ± 0.30	0.37 ± 0.19	0.75 ± 0.45	0.71 ± 0.63	0.12 ± 0.022
1	1 ± 0.019	1 ± 0.017	1 ± 0.11	1 ± 0.20	1 ± 0.12	1 ± 0.10	1 ± 0.018
10	14 ± 0.680	10 ± 0.35	242 ± 206	83 ± 52	15 ± 5.5	48 ± 1.0	10 ± 1.1

(1)CT method assuming constant amplification efficiency equal to 1 [12].

(2) CT method with amplification efficiency estimated from a dilution series [14].

(3) Amplification efficiency estimated at two product yield thresholds [15].

(4) Amplification efficiency estimated with LinRegPCR software [13].

(5) Amplification efficiency estimated with the Tichopad et.al. approach [16].

(6) Amplification efficiency estimated from the model proposed by Liu et.al. [17].

(7) Our model based real-time PCR analysis method (MoBPA).