Fig. 2.

Par-1b null mice are resistant to weight gain and are hypermetabolic on a high-fat diet. Par-1b wild-type and null males (n = 5 per genotype) were fed a high-fat diet for 16 weeks starting at 3 weeks of age. (A) Body weight of Par-1b wild-type and null mice during the high-fat diet. Averages are plotted ± standard error. All data points represent statistical differences between wild-type and null mice with P < 0.05. In an independent experiment, male Par-1b null (filled bars) and wild-type mice (open bars) (n = 8–12 per genotype) were fed a high-fat diet for 8 weeks, followed by analysis. Par-1b null mice exhibit increased metabolic rate (B), energy expenditure (C), and food intake (D) on a high-fat diet. (E) Respiratory quotient (VCO₂/VO₂) of Par-1b null mice is not statistically different from wild-type controls. Student t test P values are indicated.