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. 2007 Mar 16;104(13):5312–5317. doi: 10.1073/pnas.0701062104

Fig. 4.

Fig. 4.

Processivity of gp5/trx and gp4 on dsDNA. (A) The M13 dsDNA or (B) minicircle DNA template was preincubated with gp5/trx and gp4 as described in Materials and Methods. Reactions were initiated with MgCl2, dNTPs and [α-33P]dATP in the absence or presence of (dC)200·oligo(dG)20 to trap any free enzyme that dissociates from the circular DNA. Aliquots were removed from the reaction at the indicated times, and DNA synthesis was stopped by the addition of EDTA before separation of the products on a 0.8% agarose gel. In the control experiment, the trap was added during the preincubation step.