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Disruption of IFs in melanophores. Endogenous vimentin was stained with an antibody that binds to wild-type vimentin but not to truncated dominant-negative vimentin, and with Texas red-tagged secondary antibody (red channel). Dominant-negative or wild-type GFP-vimentin was visualized in the green channel. (a) GFP-vimentin copolymerizes with endogenous vimentin. (b) No vimentin filaments are found in cells expressing dominant-negative vimentin.
