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bFIONA traces of myosin V-driven melanosomes. (a) Traces with different rise times. Grids are 36 nm apart. (Inset) Step-size histogram of myosin V (mean: 35.1 nm, SD: 9.1 nm). The solid lines represent five-point adjacent smoothing. (b) Pairwise distribution of actin-dependent motion (taken over an adjacent averaging smoothed version of the trace). Multi-Gaussian fitting gives peaks at multiples of ≈35 nm.
