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. 2007 Mar 19;104(13):5668–5673. doi: 10.1073/pnas.0701331104

Fig. 1.

Fig. 1.

IQ-1 maintains undifferentiated state of ESCs. (a) Structure of IQ-1. (b) IQ-1 dose-dependently maintains alkaline phosphatase activity. Quantification of alkaline phosphatase activity in ESCs in a feeder-free system was performed as described in Experimental Procedures. (c) IQ-1 maintains SSEA-1 expression dose-dependently. Quantification of SSEA-1 expression in ESCs, in a feeder-free system and supplemented with either IQ-1 or LIF, was assayed as described in Experimental Procedures. SSEA-1 expression was analyzed 7 days after addition of IQ-1. (d) IQ-1 enabled ESCs to proliferate in the undifferentiated state for at least 65 days, without MEF feeders or LIF. ESCs, in media supplemented with 4 μg/ml IQ-1, are passaged 2–3 times every week at 1 × 105 to 1 × 106 cells per 6-cm dish and counted. All error bars represent ±SD. (e) Alkaline phosphatase staining of ESCs grown in a feeder-free system in the presence or absence of IQ-1 (4 μg/ml) for 65 days.