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. Author manuscript; available in PMC: 2007 Mar 28.
Published in final edited form as: J Biol Chem. 2006 Sep 19;281(48):37091–37101. doi: 10.1074/jbc.M608610200

FIGURE 3.

FIGURE 3

Affinities of the PX domain for C4-PtdIns(3)P and DHPC/CHAPS micelles. Titration of C4-PtdIns(3)P (a and b) and DHPC/CHAPS (c and d) into the PX domain induces changes in backbone 15N and 1H resonances, as observed in 1H-15N HSQC spectra. The 15N-labeled PX domain was initially either unbound (a and c), micelle-associated (b), or C4-PtdIns(3)P-bound (d). The estimated binding affinities are shown in the inset. e, model of sequential interactions of the PX domain with C4-PtdIns(3)P or DHPC/CHAPS micelles with monovalent affinities indicated.