Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Apr 1;21(7):835–847. doi: 10.1101/gad.1516207

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, Cold Spring Harbor Laboratory Press

PMC Copyright notice

Figure 1. — Ubiquitylation of H2B is present in S. pombe and is required for H3K4me and for normal growth. (A) Either the htb1-Flag or htb1-K119R-Flag strain was crossed to an untagged wild-type strain; tetrads were dissected on YES media. Shown are three tetrads from each cross. The slow-growing colonies in the htb1-K119R-Flag cross were all G418-resistant. (B) Anti-Flag Western blots on whole-cell extracts prepared from an untagged strain (htb1⁺) and the indicated tagged strains. Bands corresponding to H2B-Flag and its ubiquitylated form are indicated on the right. On the left are molecular weight markers (in kilodaltons). The asterisk denotes a probable H2B–SUMO conjugate. (C) Extracts from B were probed with the indicated antibodies.