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. 2007 Mar;14(3):126–133. doi: 10.1101/lm.496007

Figure 2.

Figure 2.

Drug condition correlation of cAMP expression visualized by immunohistochemistry. Immunohistochemical label of cAMP in the olfactory bulbs of pups exposed to peppermint odor paired with either saline (A,B), 2 mg/kg Iso (C,D), or 6 mg/kg Iso (E,F). (B,D,F) Higher magnifications of regions indicated in A, C, and E, respectively. cAMP expression was especially evident in mitral cells in B, D, and F and tended to increase in density with the higher isoproterenol dose. Scale markers in A, C, and E: 200 μm; in B, D, and F: 25 μm. (G) The percentage (%) threshold of cAMP immunohistochemical staining of mitral cells. The 2 mg/kg Iso + odor group expressed significantly higher cAMP than the saline + odor group. cAMP in the 6 mg/kg Iso + odor group was significantly higher than either the 2 mg/kg Iso + odor group or the saline + odor group. One-way ANOVA F(2,18) = 15.92, P < 0.001. The measurements were obtained from olfactory bulbs of animals sacrificed at 10 min after odor presentation. N = 7 for each group. (Iso) Isoproterenol, (EPl) external plexiform layer, (Mi) mitral cell layer, (GC) granule cell layer.