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. 2007 Mar;9(3):184–191. doi: 10.1593/neo.06841

Figure 4.

Figure 4

GRP increases Ets1 DNA binding and transcription activity. (A) Nuclear protein was extracted from cells treated with GRP (10-7 M) for 4 hours. Nuclear protein (2.5 µg/lane) was added in binding reactions, and protein/DNA complexes were resolved in a 6% DNA retardation gel. (B) SK-N-SH cells were cotransfected with 5x GAL4-Luc plus GAL4-Ets1 (WT) or GAL4-Ets1 (T38A) (dominant-negative mutant vector). Cells were treated with GRP (10-7 M) for 24 hours after serum starvation. Ets luciferase activity was significantly increased after GRP treatment (data represent triplicate determinations; mean ± SEM; *P < .05 vs control vector).