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. 2007 Mar;9(3):254–259. doi: 10.1593/neo.06805

Figure 3.

Figure 3

Normalized ProteinChip Array profiles of the immunodepletion assay from a serum probe. To confirm the affiliation of TTR with the differentially expressed protein with a molecular mass of 13,746 Da, immunodepletion was performed. An anti-human prealbumin antibody was coupled to protein A agarose and, after serum incubation, the supernatant was loaded on an IMAC30 Ni ProteinChip. Thereby, four proteins were depleted; all of them represent TTR or TTR modifications (13.74 kDa, TTR; 13.87 kDa, cysteinylated TTR; 13.92 kDa, CysGly TTR; 14.08 kDa, glutathionylated TTR). From left to right: 13.74, 13.87, 13.92, and 14.08 kDa.