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EMSA of HU-DNA. Five nanomolar relaxed plasmid samples were treated with wild-type α+β+ (a) or mutant α+β-E38A (b) HU at 0, 1.25, 1.39, 1.56, 1.79, 2.08, 2.50, 3.13, 4.17, 6.25, 12.5, 25.0, and 50 μM concentrations (lanes 1–13, respectively).
