Table 1.

Best-fit parameters of the nucleotide-induced fluorescence quenching in βY331W-εdEcF₁

Nucleotide	Additions		Estimated
	Sulfate, mM	Phosphate, mM	Kd,1, μM	Q1, %	Kd,2, μM	Q2, %
ADP*	−	−	0.043 ± 0.007	23.4 ± 0.9	37 ± 8	24.4 ± 1.2
ADP*	2	−	1.8 ± 0.6	21.7 ± 4	36 ± 12	27 ± 4
ADP†	20	−	37 ± 3‡	46 ± 1§
ADP*	−	5	2.4 ± 0.6	27.1 ± 3.5	60 ± 20	25.5 ± 3.2
MgADP*	−	−	0.01 ± 0.002	22.7 ± 0.7	8.1 ± 1.3	23.7 ± 0.8
MgADP*	2	−	0.35 ± 0.2	21.8 ± 8	4.7 ± 2.6	24.7 ± 8
MgADP*	20	−	1.8 ± 0.3	22.8 ± 2.5	28 ± 5	27.2 ± 2.4
ATP*	−	−	0.185 ± 0.03	25 ± 1	200 ± 60	23.4 ± 1.8
ATP*	2	−	7.5 ± 2	22.5 ± 5	100 ± 30	25.8 ± 4.6

*Fit to Eq. 2.

^†Fit to Eq. 3.

^‡K_d from Eq. 3.

^§Q_t from Eq. 3.