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13C NMR spectra of a liver from a fed mouse showing the disappearance of glycogen after glucagon administration. The resonance at 100 ppm is distinctive of C1 of glycogen, whereas the resonance near 130 ppm is from unsaturated fats in liver (used here an internal standard only). The plot shows changes in intensity of the C1 glycogen resonance after addition of glucagon (n = 3).
