Fig. 1.

PhysO₂ maintain the intracellular redox state closer to in vivo levels than atmosO₂. Freshly prepared negatively enriched human peripheral blood T cells (see Materials and Methods) were cultured at physO₂ and atmosO₂ oxygen for 3 days without exogenous stimulation. iGSH and iGSSG were measured by tandem MS at the beginning and on day 3 of culture. (Top) iGSH levels on day 3 expressed as percentage of iGSH on day 0. (Middle) iGSSG levels on day 3. (Bottom) Intracellular redox state (iGSH/iGSSG) on day 3. Statistics were calculated by using JMP software by least-square fit model with sample and oxygen as independent variables (see Materials and Methods). Each set of connected points represents one subject (n = 6).