Fig. 2.

iGSH and iNO levels at physO₂ are maintained closer to the in vivo levels compared with atmosO₂. Freshly prepared human PBMCs were cultured for 3 days at physO₂ and atmosO₂ without exogenous stimulation. iGSH and iNO were measured at the beginning and the end of the 3-day culture by FACS (see Materials and Methods). (Upper) CD4 T cell iGSH on day 3 expressed as fraction of iGSH on day 0 (percentage of day 0 iGSH). (Lower) CD4 T cell iNO on day 3 expressed as fraction of iNO on day 0 (percentage of day 0 iNO). Statistics were calculated by using JMP software by least-square fit model with sample and oxygen as independent variables (see Materials and Methods). Each set of connected points represents one subject. n = 16 for iGSH and n = 10 for iNO.