Fig. 3.

CD3/CD28-stimulated T cell proliferation is higher at atmosO₂ than physO₂. CFSE-stained human PBMCs were stimulated for 3 days with plate-bound CD3 (1 μg/ml) and CD28 (2 μg/ml). Cell counts were performed by using BD Trucount tubes. Proliferative index (PI) and fold change in the live CD4 T cell number was calculated as described in Materials and Methods. (Upper) Higher proliferation index (1.63 ± 0.17 at atmosO₂ versus 1.32 ± 0.09 at physO₂). (Lower) Significantly higher fold increase (2.34 ± 0.69 versus 1.47 ± 0.52) in CD4 T cells stimulated at atmosO₂ vs. physO₂. Statistics were calculated by using JMP software by least-square fit model with sample and oxygen as independent variables. Each set of connected points represents one subject (n = 16).