Fig. 2.

Calcium and cAMP pathways activate CREB synergistically in a calcineurin-dependent manner. (A) Effect of FSK and KCl on mouse cortical neurons transfected with a CRE-luciferase reporter in the absence (Veh.) or in the presence of CsA. Results are displayed as the mean (±SEM) of fold luciferase activity (n = 3) relative to control. ∗, Significantly different from all of the other conditions (two-way ANOVA followed by Bonferroni's post hoc test; P < 0.05). (B) Western blot of phospho-(Ser-133) CREB (pCREB) and total CREB levels in cortical neurons exposed for 30 min to FSK and/or KCl. (C) Mean densitometric band analysis of the Western blots shown in B. Data are representative of triplicate determinations. Intensity values were normalized to the FSK plus KCl condition, which was set equal to 100%.