Fig. 2.
Immunoblot analysis of brain homogenates from mice inoculated with PrP-21 and PrP-8. (A) Lanes 1 and 2, Tg 101LL-21 (mouse with neuropathologically confirmed prion disease, 277 days postinoculation); lanes 3 and 4, Tg 101LL-8s (mouse with neuropathologically confirmed prion disease, 622 days postinoculation); and lanes 5 and 6, Tg 101LL-8a (asymptomatic mouse, without spongiform degeneration showing multicentric amyloid plaques, 703 days postinoculation). Samples in lanes 2, 4, and 6 were treated with 20 μg/ml PK. The film was overexposed to show the low levels of PK-res PrPSc present in the samples shown in lanes 2 and 4. (B) Low levels of PrPSc were identified in one 101LL-8a mouse after treatment with 20 μg/ml PK. PrP was detected with mAb 7A12.