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. 2007 Mar 6;104(11):4712–4717. doi: 10.1073/pnas.0609241104

Fig. 3.

Fig. 3.

Cold-PK digestion of brain homogenate from Tg 101LL-8 mice and controls. Lane 1, Tg 101LL-8a (asymptomatic mouse with multiple PrP-amyloid plaques, 633 days postinoculation); lane 2, Tg 101LL-8a (asymptomatic mouse with multiple PrP-amyloid plaques, 716 days postinoculation); lane 3, uninfected Tg 101LL mouse; lane 4, uninfected 129/Ola control mouse; and lane 5, ME7-scrapie-infected 129/Ola control mouse. The 22- to 24-kDa PrP band (corresponding to “cold PK”-resistant fragment of PK-sensitive PrPSc) is present only in the ME7 scrapie-infected control mouse. All samples were treated with 250 μg/ml PK on ice for 1 h and deglycosylated with PNGase F. ME7 scrapie control was loaded at ≈25% of the concentration of lanes 1–4 to allow comparison. The blot was probed with mAb 7A12. The image was cropped from a single blot to remove lanes with irrelevant samples.