Fig. 3.

Immunoelectron microscopy for Mda1. Ultra-thin sections were labeled with an anti-Mda1 rabbit antibody and a 10 nm gold particle-conjugated secondary antibody, and were observed under transmission electron microscopy. (A) One of the five serial sections showing the entire cell at the early stage of mitochondrial division. (B) Cross-sectioned dividing mitochondria with electron-dense images of sectioned MD ring pinching the constriction site. (C) Section showing a cell at the final stage of mitochondrial division. (D) Three-fold magnification of C, showing Mda1 signals around a constricted MD ring. (E) The five panels on the Left are images of the five serial sections showing the belt localization of Mda1 around the mitochondrion, one of which is in A. The Right end panel shows signals in the five superimposed left images. N, nucleus; m, mitochondrion; mb, microbody; cp, chloroplast. [Scale bars: 200 nm (A and B) and 500 nm (C).]