Fig. 4.

Disassembly of the mitochondrial division machinery. The sets of two lanes are fractions from S/G₂-arrest and M-arrest. Pellets 35P and 20P in Fig. 1 were combined and subsequently extracted with 2 mM GDP (GDP), 2 mM GTP (GTP), 2% heptylthioglucoside (HTG), and 1 M Tris·HCl plus 5%heptylthioglucoside (TrH). Proteins from extracts and pellets (P) were resolved by 10% SDS/PAGE. The molecular weight marker (m) is indicated on the right. Filled arrowheads indicate Dnm2; open arrowheads indicate Dnm1. Arrows indicate Mda1.