Fig. 2.

Function of HsMetAP1 is required for accurate cell cycle progression through G₂/M phase. (A) FACS cell cycle analysis for unsynchronized HeLa cells treated with 1 for 24 h. (B) Synchronized HeLa cells showed delayed G₂/M progression in the presence of compound 1. Double thymidine synchronized cells were released for 6, 9, and 12 h, respectively, before being collected for FACS analysis. (C) Western blot analysis from HeLa cells treated with respective siRNA duplexes for 48 h. Blots were sequentially probed with anti-HsMetAP1, HsMetAP2, and 14-3-3γ proteins; β-actin is the gel-loading control. (D) HsMetAP1 siRNA duplexes delayed cell cycle progression during G₂/M phase. Synchronized HeLa cells were harvested for FACS analysis at different time points from double thymidine release.