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Inhibition of HsMetAP1 resulted in delayed degradation of cyclin B protein. (A) A significant delay of cyclin B1 degradation in the presence of 1 is shown by Western blot. Cell lysates were harvested at different time points from double thymidine release. (B) RT-PCR analysis of the cyclin B1 mRNA level was carried out from total RNA isolated at different time points with appropriate primers specific for cyclin B1 and β-actin.
