Fig. 4.

HsMetAP1 inhibition induces cellular apoptosis. (A) Ethidium bromide-stained genomic DNA isolated from Jurkat T cells in the absence (−) and presence (+) of 10 μM compound 1, respectively, after 16 h of treatment. M, marker. (B) Western blot analysis of protein lysate isolated from vehicle- and compound 1-treated Jurkat T cells for 24 h. Blots were sequentially probed with antibodies specific to PARP, procaspase-3, and active caspase-3. An arrow indicates the release of the 89-kDa fragment from PARP protein. β-Actin is the gel-loading control.