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. 2006 Nov 20;103(48):18220–18225. doi: 10.1073/pnas.0605131103

Fig. 4.

Fig. 4.

Tpn preferentially binds empty class I molecules. (A–C) Silver-stained SDS/PAGE showing direct protein loads of A2 (A, lane 1, 0.3 μg), B35 (C, lane 2, 0.2 μg), or sTpn (A, lane 2, 0.05 μg; B, lane 1, 0.2 μg; C, lane 1, 0.1 μg) or anti-FLAG IP (A, lanes 3 and 4; B, lanes 2 and 3; C, lanes 3–6) of indicated proteins (3 μM each) after incubations at 30°C for 6 h. Empty class I molecules are represented as eA2 and eB35, and untreated class I molecules are represented as A2 and B35. Data are representative of two (A and B) or three (C) independent analyses.