Fig. 3.

Overexpression of miRNAs in primary cardiomyocytes. (A) Primary rat cardiomyocytes in serum-free medium (SF) were infected with adenoviruses expressing individual miRNAs at the indicated MOI. miRNAs were detected by Northern blot analysis on 10 μg of RNA. Positions of the pre-miRNAs and mature miRNAs are indicated. (B) Actinin staining of cardiomyocytes shows that adenoviral overexpression of several miRNAs induces morphological changes characteristic of hypertrophy. Cells were infected with pAd β-gal as a negative control. As a positive control, cells were stimulated with phenylephrine (PE), a potent inducer of hypertrophy.