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. 2006 Nov 15;103(48):18255–18260. doi: 10.1073/pnas.0608791103

Fig. 4.

Fig. 4.

Cardiac-specific overexpression of miR-195 is sufficient to drive cardiomyopathy. (A) H&E-stained sections on hearts of WT and miR-195 Tg, line 3, indicate that overexpression of miR-195 induces death 2 weeks after birth because of cardiac dilation. (Scale bar: 2 mm.) (B) Northern blot analysis on hearts from WT and miR-195 line 3, miR-195 line 1, and miR–214 Tg line 1, confirming a 29-, 26-, and 15-fold cardiac-specific miRNA overexpression, respectively. (C) H&E-stained sections show that overexpression of miR-195 induces cardiac growth at 2 weeks of age, which within 6 weeks progresses to a dilated phenotype, whereas cardiac overexpression of miR-214 has no phenotypic effect. The bottom two panels show a high magnification of the H&E section, indicating severe myocyte disorganization in the miR-195 Tg animals compared with WT. (Scale bars: Top and Middle, 2 mm; Bottom, 20 μm.) (D) H&E-stained sections show that overexpression of miR-214 has no morphological effect on the heart.