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. 2006 Nov 14;103(48):18362–18367. doi: 10.1073/pnas.0606449103

Fig. 3.

Fig. 3.

Both tRNA import and tRNA binding are inhibited by TOM20 and TOM40 antibodies. (A) In vitro import of tRNA into isolated mitochondria. Labeled in vitro-transcribed tRNAAla (105 cpm) was incubated with isolated mitochondria in the absence (−) or presence (+) of 5 μl of antibodies raised against VDAC, TOM20, TOM40, LeuRS, or NAD9. The corresponding ethidium bromide-stained gels are shown below the autoradiograms. In, input RNAs (1,000 cpm). Incubation of tRNAAla transcript with mitochondria in the presence of increasing concentration of TOM20 and TOM40 antibodies shows that 1 μl of the antibodies completely inhibits import (data not shown). (B) Binding of tRNA to isolated mitochondria. Binding was performed as described (14). Incubations were performed in the absence (−) or presence (+) of 5 mM ATP. Note that between A and B, exposures times for the autoradiography are very different (≈10 times longer exposure for import than for binding).