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. 2007 Mar 28;104(14):6049–6054. doi: 10.1073/pnas.0701240104

Fig. 2.

Fig. 2.

Caspase activation in E. huxleyi by EhV1 infection. (A) Caspase-specific activity measured by the cleavage of IETD-AFC in Ehux374 and Ehux373 cell extracts. Error bars represent standard deviations among triplicate measurements. Shaded region represents the 60-h infection window. (B) Caspase activity for Ehux373 cell extracts (in A) plotted on a different scale to illustrate activity increases in dying batch cultures (t > 158 h). (C) Epifluorescence micrographs showing fluorescence of Ehux374 attributable to cellular chlorophyll (Upper) and staining with CaspACE FITC-VAD-FMK (Lower). Identical fields containing two cells are pictured (arrows). Note that the bright autofluorescent cell has minimal staining with CaspACE, whereas the dim autofluorescent (i.e., photosynthetically unhealthy) cell displays much brighter CaspACE staining. (Scale bars: 5 μm.) (D) Plots of the fluorescence distribution for CaspACE-stained control and EhV1-infected cell populations collected at 0 h and 56 h after infection and measured by flow cytometry (5,000 cells counted); inset lines designate the percentage of cells associated with each gate position.