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. 2007 Mar 28;104(14):6049–6054. doi: 10.1073/pnas.0701240104

Fig. 3.

Fig. 3.

Detection of metacaspase expression in E. huxleyi. (A) Western blot analysis of Ehux374 (Left) and Ehux373 (Right) cell extracts collected during a time course of viral infection, showing immunohybridization to polyclonal antibodies generated against a purified, recombinant E. huxleyi metacaspase protein (EhMC). Lane numbers represent collection time (hours after infection). Cell extracts correspond to samples presented in Figs. 1 and 2. (B) Western blot analysis testing immunohybridization of purified, recombinant human caspase 8 (10 ng per lane) to the following antisera: EhMC, polyclonal antisera generated against a purified, recombinant EhMC; Pre, preimmune serum collected before injection of EhMC; Rub, polyclonal antisera generated against purified RuBisCo. Banding is consistent with the expected ≈18-kDa subunit for caspase 8, according to the manufacturer. Molecular masses (in kilodaltons) are indicated.