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. 2007 Apr 2;117(4):1068–1077. doi: 10.1172/JCI30117

Figure 2. Inactivation of Hif-2α suppresses the development of polycythemia in PEPCK-Vhlh mutant mice.

Figure 2

(A) Photograph of centrifuged microcapillary tubes containing blood from representative PEPCK-Vhlh (hematocrit 89%; 1), PEPCK-Vhlh/Hif-2α (hematocrit 48%; 2), and control (hematocrit 53%; 3) mice. (B and C) Elevated hemoglobin (Hgb) and red blood cell numbers in PEPCK-Vhlh mutant mice were suppressed in PEPCK-Vhlh/Hif-2α mutant mice. Shown are hemoglobin concentrations and red blood cell (rbc) numbers in blood collected from PEPCK-Cre mutant and control (Cre–) mice determined by a complete blood count analyzer. Shown are the mean values for 6 individual mice. Error bars represent SEM. (D) Inactivation of Hif-2α significantly decreases Epo transcript levels in PEPCK-Vhlh mutant livers. Shown are relative Epo mRNA transcript levels normalized to 18S in the livers of PEPCK-Cre mutant and Cre-negative mice. Bars represent mean mRNA transcript levels of 3 mice per group. Error bars represent SEM. **P < 0.001 compared with PEPCK-Vhlh as determined by Student’s t test.