(A) Identification of β-catenin as a Glis2-interacting protein by mammalian two-hybrid analysis. HCT116 cells were co-transfected with (UAS)5-LUC, pRL-SV40, pM, pVP16, pM-Glis2, and increasing amounts of pVP16-β-catenin as indicated. After 48 h, cells were harvested and assayed for reporter activities. The relative LUC activity was calculated and plotted.