Figure 1.

NHERF-2 is a potential binding partner for β-catenin. Schematic diagrams of β-catenin (A) and NHERF-2 (B). Highlighted are the portion of β-catenin used as bait for phage display and the portion of NHERF-2 that bound to β-catenin in phage display as well as the potential PDZ-binding motif (WFDTDL) in last six AA of β-catenin and the ERM-binding site at the C terminus of NHERF-2. (C) Purified GST fusion proteins including various NHERF-2 domains (or NHERF-1 as a negative control) were resolved by SDS-PAGE and immunoblotted with anti-GST antibodies, mAb 32B6, and mAb 33E2. Highlighted in B are the regions of NHERF-2 recognized by the two mAbs.