Figure 10.

The N-cadherin/β-catenin/NHERF/PDGF-R complex contributes to HeLa cell migration. (A) HeLa cells expressing PDGF-Rβ, HeLa cells expressing PDGF-Rβ together with shRNA against N-cadherin (N-cadherin KD), or HeLa cells expressing PDGF-Rβ together with shRNA against NHERF-1 (NHERF-1 KD) were serum starved for 24 h, scratched with a sterile pipette tip, and treated with (second 3 panels) or without (first 3 panels) PDGF-BB ligand. The right two panels show HeLa cells expressing PDGF-Rβ together with shRNA against NHERF-1 and full-length NHERF-2, or PDGF-Rβ together with shRNA against NHERF-1 and the NHERF-2 PDZ I domain alone. Cells were allowed to migrate into the denuded area, and phase micrographs taken at 0, 18, and 36 h. (B) HeLa cells expressing PDGF-Rβ were extracted, immunoprecipitated with antibodies against N-cadherin, and immunoblotted for PDGF-Rβ (left) or immunoprecipitated with antibodies against PDGF-Rβ and immunoblotted for N-cadherin and β-catenin (right). On both gels, lane 1 is cell extract and lanes 2 and 3 are the immunoprecipitation reactions.